Fig. 5. Anisomycin treatment activates p38 in an anchorage-independent manner.
Serum-starved detached NIH 3T3 cells were either maintained in suspension
(Sus) or plated onto fibronectin (Fn)-coated dishes for 2 hours in DMEM/BSA.
(A) At the 2 hour time point, cells were stimulated appropriately with
increasing doses of anisomycin (0, 25, 50 and 200 ng/ml) for the 30 minutes.
(B) Cells were treated with 50 ng/ml anisomycin for the times indicated. Under
each condition, cells were lysed in modified RIPA buffer and cell lysates
probed with antibodies to phosphorylated and total p38.
