Fig. 4. The majority of the trypsin-resistant SNAREs are not in SDS-resistant
complexes. Chromaffin cells were permeabilised and exposed for 15 minutes to
various [Ca2+], then maintained for a further 30 minutes in the
absence (A) or presence (B) of 100 µg/ml trypsin, before being harvested in
the presence of 2 mM PMSF and their membranes isolated. The samples were
solubilised in SDS-PAGE sample buffer and split into two; one of each was
boiled for 2 minutes. Boiled and non-boiled samples were subjected to SDS-PAGE
and western blotting.
