Fig. 1. GFP-Cdc16 and GFP-Cdc27 appear to be incorporated into the endogenous APC/C. (A) A western blot of wild-type (WT) (lanes 1,3), GFP-Cdc16 (lane 2) and GFP-Cdc27 (lane 4) expressing embryos probed with anti-Cdc16 antibodies (lanes 1,2) or anti-Cdc27 antibodies (lanes 3,4). Twenty 0-4-hour-old embryos were loaded per lane, and blots were stained with anti-tubulin antibodies to confirm an equal loading of protein (not shown). (B) Embryo extracts from wildtype (panels 1,2), GFP-Cdc16- (panel 3) or GFP-Cdc27- (panel 4) expressing embryos were separated on a Superose 6 gel filtration column and fractions were probed with anti-Cdc27 (panel 1), anti-Cdc16 (panel 2) or anti-GFP (panels 3,4) antibodies. The position of standard molecular weight markers is indicated above the blots. (C) GFP antibodies (lanes 1,3) or Random rabbit IgG (lanes 2,4) were used to immunoprecipitate proteins from embryos expressing GFP-Cdc16 (lanes 1,2) or GFP-Cdc27 (lanes 3,4). Blots were probed with anti-GFP (top panels), anti-Cdc16 (middle panels) or anti-Cdc27 antibodies (bottom panels).

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