Fig. 4. The mitotic arrest induced in Cdc16^RNAi and Cdc27^RNAi cells is morphologically distinct. (A) A graph showing the mitotic index of mock-, Cdc16^RNAi-, Cdc27^RNAi- or Cdc16^RNAi- + Cdc27^RNAi-treated S2 cells. This was calculated by counting the percentage of cells in mitosis as judged by phospho-histone H3 staining. Error bars represent the standard deviation. Results were pooled from four different experiments. (B) A low magnification view of Cdc16^RNAi- (top panel) or Cdc27^RNAi- (bottom panel) treated cells stained to reveal the distribution of DNA (red), phosphohistone H3 (blue) and microtubules (green). Note how most of the mitotic Cdc16^RNAi cells have their chromosomes roughly aligned at the equator of a metaphase-like spindle, whereas most of the mitotic Cdc27^RNAi cells have elongated spindles that are in a much more disorganised state. Bar, 10 µm. (C) A graph showing the proportions of mitotic cells in different states of mitosis. Typical examples of each mitotic state are shown underneath each graph; the colours are the same as in B: chromosomes aligned on a metaphase-like spindle (panel 1); chromosomes spread throughout an elongated spindle (panel 2); condensed and decondensed chromatin (which appears red, as it is not stained by the anti-phosphohistone H3 antibody) in the same cell (panel 3); a telophase-like cell (panel 4). Error bars represent the standard deviation. Bar, 5 µm.

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