Fig. 5. The distribution of the anti-centromere antibody CID in Cdc16^RNAi and Cdc27^RNAi cells. Control (A,B) or Cdc16^RNAi or Cdc27^RNAi (C-G) cells were stained to reveal the distribution of CID (red), DNA (blue) and microtubules (green). All the images shown are projections of several serial sections that were taken through the entire cell. In control cells during metaphase (A), the centromeres were aligned in closely spaced pairs on the metaphase plate; these pairs were clearly separated by early anaphase (B). In Cdc16^RNAi or Cdc27^RNAi cells in which the majority of the chromosomes appeared to be roughly aligned on a metaphase plate (C-E), the behaviour of the centromeres fell into three classes (the percentage of cells in each class for each RNAi treatment are shown beneath; C-E). Centromeres were either aligned in closely spaced pairs (C) or were aligned as pairs that appeared to have separated to some extent (D) or were more randomly distributed on the spindle (E). In Cdc16^RNAi or Cdc27^RNAi cells in which the spindles were elongated (F,G), the centromeres were spread throughout the spindle region. The arrows in F shows a region where it appears that sister chromatids are being pulled apart by tension on the spindle. Bar, 5 µm.

Return to article