Fig. 7. An analysis of the levels of various cell cycle proteins in Cdc16^RNAi and Cdc27^RNAi cells. The western blots show the levels of Cdc16 (panel 1), Cdc27 (panel 2), Cyclin A (panel 3), Cyclin B (panel 4) and Fzy (panel 5) in mock-treated (-) or RNAi-treated (+) cells. All blots were also probed with anti-tubulin antibodies to confirm an equal loading (data not shown), and we show the levels of a non-specific band that is variably recognised by the anti-Cdc16 antibodies (asterisk, panel 1). A slower migrating form of Cyclin A is highlighted with an arrowhead in the Cdc27^RNAi- and Cdc16^RNAi- and Cdc27^RNAi-treated cells.

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