Fig. 2. mRNA and protein expression analysis of EFA6-like genes. Poly(A)⁺ RNA from the indicated human tissues was hybridised with probes derived from EFA6A (A), EFA6B (B) and EFA6C (C) cDNAs. The blots were also probed with ß-actin cDNA to ensure that loading was equal between the different tissues (data not shown). H, heart; B, brain; P, placenta; L, lung; Li, liver; S, skeletal muscle; K, kidney; Pa, pancreas; Sp, spleen; T, thymus; Pr, prostate; Te, testis; O, ovary; Si, small intestine; C, colon; PB, peripheral blood. D shows immunoprecipitations from lysates of Jurkat human T lymphocytes (lanes 1 and 5), BHK cells (lanes 2 and 6) or BHK cells overexpressing N-terminally VSV-G-tagged EFA6B (lanes 3, 4, 7) using anti-EFA6B (lanes 1-3), anti VSV-G tag (lane 4) or anti-Sec10 irrelevant antibodies (lanes 5-7). EFA6B and VSVG-EFA6B in the immunoprecipitates were detected with anti-EFA6B antibodies (lanes 1-3 and 5-7). Alternatively, VSVG-EFA6B was detected with an anti-VSVG antibody (lane 4). EFA6B (predicted molecular weight 116.34 kDa) migrates as a 180 kDa species in SDS-PAGE (arrows). Asterisks denote heavy (H) and light (L) immunoglobulin chains.

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