Fig. 6. Effect of EFA6A and EFA6B variants on cortical actin cytoskeleton morphology. BHK cells were transfected with EGFP-tagged EFA6A (A-C); EFA6A variants corresponding to the isolated PH domain (EFA6A-PH, D-F); the C-terminal region including the PH domain (EFA6A-PHCTer, G-I); the C-terminal region with the PH domain deleted (EFA6A-CTer, J-L), EFA6B (M-O); or the C-terminal region and adjacent PH domain of EFA6B (P-R). After fixation, cells were stained with Texas-Red-conjugated phalloidin to visualize actin filaments (B,E,H,K,N,Q). Localization of EGFP constructs is shown in panels A,D,G,J,M,P. Panels C,F,I,L,O,R show the superimposed images (EGFP constructs are show in green, and F-actin is shown in red). Confocal sections were taken in the dorsal plane of the cells. Bar, 10 µm.

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