Fig. 4. Labeling of contractile vacuole and endosomal membranes with VatM-GFP. Dictyostelium cells expressing VatM-GFP were observed following a 70 minute incubation in one-third strength nutrient medium containing TRITC-dextran (2 mg/ml). Three signals were recorded: green (VatM-GFP), red (TRITC-dextran), and blue (phase contrast). All three channels are shown in A and B, whereas C shows the same image as B minus the red channel. A is a focal plane near the substratum showing the contractile vacuole complex, and B and C are a focal plane higher in the cell, showing several endosomes and also some elements of the contractile vacuole complex including several vacuoles, which are phase lucent. The arrows mark endosomes whose periphery is not labeled with VatM-GFP (presumptive late endosomes). Bar, 10 µm.

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