Fig. 7. Time course of VatM-GFP delivery to a new phagosome. This cell already contained several yeast particles in a multi-particle phagosome whose membrane was strongly labeled with VatM-GFP. The cell had been pre-labeled with TRITC-dextran, most of which was also localized in this large phagosomal compartment. The paucity of free endosomes correlated with slow delivery of VatM-GFP to the new phagosome. (A) Uptake of the new yeast particle; (B) 3 minutes and 15 seconds after uptake; (C) 6 minutes and 20 seconds after uptake; (D) 9 minutes after uptake. In C and D, TRITC-dextran is visible at the bud neck of the new phagosome and also at a point of contact between the new phagosome and the pre-existing phagosomal compartment, suggesting that fusion has occurred. Bar, 10 µm. (These frames are taken from Movie 2.)

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