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Fig. 2. Mutant opsin does not accumulate in the Golgi. Cells expressing WT (WT-GFP)
and mutant (P23H-GFP) opsin tagged with GFP were treated with BFA (20 µg/ml
for 15 minutes prior to fixation). BFA dispersed the Golgi (ß-COP) and
the WT-GFP opsin that was trafficking through the Golgi but did not disperse
the P23H-GFP opsin from its juxtanuclear position. Bar, 10 µm.
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