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Fig. 7. Male chromosomes condense and segregate at mitosis in the absence of
AKAP95. (A) The FPN of normal fertilized PN stage embryos was mechanically
removed and (B) resulting embryos were allowed to enter mitosis and (C) cleave
to the two-cell stage. Indicated stages were examined by DNA staining with PI
(red) and immunofluorescence analysis of AKAP95 (green). Merged fluorescence
images are shown. Dotted circles delineate embryos or blastomeres. PB, polar
body. Bar, 20 µm.
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