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Fig. 2. Effect of TPA and mßCD (A) or mßCD/chol (B) on the activity of
PKC. A431 cells were washed twice with DMEM medium, serum-starved for 4 hours
and preincubated with or without 5 mM mßCD or mßCD/chol for 30
minutes at 37°C before addition of 1 µM TPA. After 10 minutes at
37°C, the PKC activity was measured using the Protein Kinase C Assay
System according to the manufacturer's instructions. The error bars show
deviations between duplicates of a typical experiment.
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