Fig. 2. Induction of Ptc receptor expression by Ihh, N-Ihh, N-Ihh W160G or exogenous N-Shh in CFK-2 cells. (A) Expression of Ptc in stably transfected CFK-2 cells. CFK-2 cells that were stably transfected with pcDNA3, Ihh-pCDNA3, N-Ihh-pcDNA3 or N-Ihh W160G-pcDNA3 were subjected to postconfluent growth conditions for a period of 16 days and total RNA was extracted intermittently at the indicated times. Ptc mRNA expression was assessed by northern blot analysis and compared to the respective transgene level. (B) A graphic depiction of the results shown in A, comparing Ptc expression with the levels of GAPDH expression where bars represent average levels at the three time points (^*P<0.01). (C) Comparison of Ptc expression to transgene levels in Ihh-, N-Ihh- and N-Ihh W160G-transfected cells after these were normalized to GAPDH mRNA. (D) Dose response induction of Ptc expression by exogenous N-Shh. CFK-2 cells were subjected to exogenous treatment with increasing concentrations of recombinant N-Shh or vehicle for a period of 6 days in postconfluent culture, after which total cellular RNA was extracted and subjected to northern blot analysis.

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