Fig. 1. Selective diversion of host cell lipids. Coverslip-grown host cells were incubated with fluorescent probes alone (hosts), infected with T. gondii prior to the introduction of the probe (toxo+lipid), or labeled with the probe before infection with T. gondii (lipid+toxo). Images of live cells were immediately acquired by confocal microscopy. Single, 0.4 µm thick optical sections are shown. Cholesterol, NBD-cholesterol; PC, BODIPY-phosphatidylcholine; PA, BODIPY-phosphatidic acid; C4C9, C4-BODIPY-C9. Red emission in the C4C9 panels corresponds to excimer shift of highly concentrated BODIPY. Intracellular parasites in the PC lipid+toxo panel are indicated with asterisks. Bars, 20 µm (hosts); 5 µm (toxo+lipid, lipid+toxo).

Return to article