Fig. 10. The C-terminal domain of NUANCE targets the NE. (A-C) Confocal optical section through the apical part of the COS7 nucleus showing that the overexpressed GFP-Cterm1 protein was arranged in patches displacing nuclear pores (arrows), detected by anti-Nup358 antibody (B). (D-F) The GFP-Cterm2 remained associated with the NE in prophase (D, arrow) when the nuclear pores begin to disassemble as indicated by diffuse anti-Nup358 staining (E). DNA was visualized with DAPI (F,I,L). (J-L) Two examples of the cellular localization of the GFP-NUA^460-6643 overexpressed in COS7 cells stained with TRITC-phalloidin (H,K). The GFP-NUA^460-6643 possesses the N-terminal ABD and the C-terminal Klarsicht-like domain but lacks most of the central rod domain. The GFP-NUA^460-6643 is arranged in fibers (G-I, small arrowheads) or perinuclear aggregates (J-L, arrows) partially colocalizing with actin. Dynamic cortical cytoskeleton (G-I, arrow) and some of the stress fibers (G-I, large arrowhead) do not recruit GFP-NUA^460-6643. (I,L) show overlays of the GFP, TRITC and DAPI channels. Bars (A-C) 5 µM; (D-F) 10 µM; (J-L) 20 µM.

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