QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                         What's this?

The mechanism of COPI vesicle formation

The COPI complex coats the surface of vesicles travelling within the Golgi and recycling back to the ER. COPI-coated vesicles can be generated from purified Golgi membranes and analysed in vitro, and this approach is providing significant insight into their biogenesis. In a Commentary on p. 3235, Walter Nickel, Britta Brügger and Felix Wieland discuss the COPI machinery and its assembly/disassembly in the context of this work. The COPI coat comprises a heptameric coatomer protein and the small GTPase ARF1. In vitro studies suggest that GDP-bound ARF1 is recruited to the Golgi membrane by a member of the p24 family termed p23. The latter dissociates from ARF1 upon GTP/GDP exchange and becomes able to bind to coatamer — perhaps through rearrangement of p23 oligomers. Nickel et al. speculate that interactions between coatomer and p23/p24 oligomers drive the polymerization of COPI that accompanies vesicle formation, proposing a model for vesicle formation in which p23, p24, ARF-GTP and coatamer constitute the minimal machinery necessary.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

Related articles in JCS:

Vesicular transport: the core machinery of COPI recruitment and budding

Walter Nickel, Britta Brügger, and Felix T. Wieland
JCS 2002 115: 3235-3240. [Abstract] [Full Text]  

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                         What's this?