Fig. 1. Adherent neuropsheres form layers of migrating cells. Phase-contrast micrographs show that, 30 minutes after adhesion of neurospheres on coated coverslips (A), few cells have started to migrate out of the sphere. 24 hours after adhesion (B), outgrowing cells formed layers (arrow) surrounding the sphere (dotted circle). After 7 days (C), the number of cells decreased in the sphere (dotted circle), which was flattened, and increased in the outgrowth. Bar, 150 µm (A); 300 µm (B,C).

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