Fig. 1. Loss of C-Nap1 staining during mitosis. (A) HeLa cells were synchronized at the indicated cell cycle stages. Equal amounts of protein were then separated by 10% SDS-PAGE and probed by immunoblotting with the antibodies indicated. (B) HeLa cells were collected in G2 or blocked in mitosis using either nocodazole or monastrol, as indicated. Equal amounts of protein were separated by 7.5% SDS-PAGE, and probed by immunoblotting. For the control, insoluble fractions were also resuspended and corresponding volumes (1:1) analyzed in parallel. (C) Mitotic HeLa cells (M) were collected by mechanical shake off and asynchronously growing cells (asy) by trypsinization. Levels of C-Nap1 were then determined by immunoblotting, using either C-Ab or M-Ab (Fry et al., 1998a; Mayor et al., 2000). All C-Nap1 signals were standardized relative to the -tubulin signal. The histogram shows the relative levels of C-Nap1 in M-phase and asynchronously growing cells.

Return to article