Fig. 2. C-Nap1 is not degraded during mitosis. (A) HeLa cells were collected in G2 or blocked in mitosis in the presence or absence of the proteasome inhibitor MG132. Equal amounts of protein were separated by 7.5% SDS-PAGE and probed by immunoblotting using the antibodies indicated. (B) Destruction assay in Xenopus egg extract. [³⁵S]-labeled cyclin B and C-Nap1 were added to a mitotic extract and samples taken at the indicated times (minutes). Proteins were separated by 10% SDS-PAGE and exposed for autoradiography.

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