Fig. 8. Active Nek2 modulates C-Nap1 patch formation. (A) Histograms illustrate the percentage of transfected cells falling into three defined categories (as defined in B), after co-transfection of mycC-Nap1 with either active Nek2 (Nek2-wt) or a catalytically inactive Nek2 mutant (Nek2-K37R). Results are shown as averages of two independent experiments. (A total of 588 cells were counted for co-transfections with Nek2-K37R and 600 cells for co-transfections with Nek2-wt.) (B) Representative examples of three types of C-Nap1 structures, used for classification of transfected cells. Class I, C-Nap1 localized to centrosomes without inducing the formation of grossly abnormal structures (a). Class II, C-Nap1 formed one predominant patch closely associated with the centrosome, with or without accompanying smaller patches (<10 patches in total) (b). Class III, C-Nap1 formed many (>10), often small, patches (speckles) (c). Bar, 10 µm (Ac).

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