Fig. 2. Tissue distribution of Stau2 proteins and transcripts. (A) Western blot analysis of Stau2 in neurons. Brain extracts were separated by SDS-PAGE and stained with anti-Stau2 antibodies. Lane 1, mouse monoclonal; lane 2, rabbit polyclonal; lane 3, mouse polyclonal. (B) Northern blot analysis of Stau2 expression in mouse tissues. mRNAs isolated from multiple tissues were transferred to a nylon membrane and hybridized with either a mouse Stau2 cDNA probe (top panel), a mouse Stau1 cDNA probe (middle panel) or an actin cDNA probe (lower panel). Lane 1, brain; lane 2, heart; lane 3, spleen; lane 4, kidney; lane 5, testis; lane 6, ovary. Blots were exposed for 16 hours. (C) RT-PCR analysis of the Stau2 transcripts in brain. Total RNA was isolated from mouse brain and RT-PCR amplified with primers flanking the alternatively spliced exon. PCR amplification was performed with different amounts of starting RNA and cycle numbers. Control RNA (1 µg, no reverse transcription step) was amplified for 32 cycles.

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