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Fig. 5. Co-immunoprecipitation of acetylated histone H4 and HDAC II with GAL4-hDaxx. 293T cells overexpressing equivalent amounts of GAL4-hDaxx, GAL4-hDaxx{Delta}1-132, GAL4-hDaxx{Delta}1-352 and GAL4-SPT (A) were lysed in the presence of protease and phosphatase inhibitors. The resulting cell lysates were immunoprecipitated with an anti-GAL4 DBD antibody followed by detection with anti-GAL4 (B), anti-acetylated histone H4 (C) or anti-HDAC II (D) antibodies. The asterisks in panel B indicate the location of the IgG heavy and light chains.





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