Fig. 6. Co-immunoprecipitation of histone deactylase activity with GAL4-hDaxx. Equal amounts (100 µg) of total cell lysates from 293T cells overexpressing equivalent levels of either GAL4-hDaxx or GAL4 alone (data not shown) were immunoprecipitated with an anti-GAL4 DBD antibody. The resulting immune complexes were analyzed for histone deacetylase activity either in the absence (gray bars) or presence (striped bars) of the specific HDAC inhibitor, sodium butyrate (see Materials and Methods). Total HeLa nuclear extracts (10 µg) were used as a positive control for the deacetylase assay, and the results are presented as the total CPM of [³H]-acetate released for each sample.

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