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Fig. 5. Morphological analysis of the degree of maturation of cell-cell contacts in
T98G and U373MG cell lines. Confocal analysis of immunofluorescence staining.
The glioblastoma cell lines were plated onto poly-L-lysine-coated glass
coverslips, fixed in 4% paraformaldehyde and double-stained for ß-catenin
and the indicated antibodies or the FITC-conjugated phalloidin to stain actin.
The arrowheads indicate mature cell-cell contacts, and the arrows indicate
immature cell-cell contacts. Bar, 10 µm.