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Fig. 5. The cysteine-rich domain of SNAP-25 is necessary to support regulated exocytosis in intact cells. (A) Ca2+-dependent secretion of ß-Gal activity in G14 cells stably expressing BoNT/E and transiently transfected with POMC-ß-Gal and pcB7 or SNAP-25A/ER-pcB7 or Delta-SNAP-25A/ER-pcB7. (B) Ca2+-dependent secretion of ß-Gal activity in G14 cells stably expressing BoNT/E and transiently transfected with POMC-ß-Gal and pcB7 or SNAP-25A/ER-pcB7 or CA-SNAP-25A/ER-pcB7. Ca2+-dependent ß-Gal release (as in Fig. 1G) is calculated from the data of four independent experiments done with triplicate samples.





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