Fig. 3. A pile-up of the Drosophila innexin family showing conserved residues in black. Putative transmembrane domains are underlined and marked as M1, M2 etc. Horizontal lines mark the breakpoints for the chimeras we tested and are labeled with the shakB(N) residue number on the left and the corresponding ogre residue number on the right. D-inx-7 rescues shakB² whereas ogre does not. In addition, the chimeric studies show that residues 97-172 of shakB(N) are the residues needed for shakB(N) specificity. In this critical region those residues where D-inx-7 is identical or chemically similar to shakB(N), but chemically different from ogre, are marked with asterisks. These marked residues are likely to be the ones that are most crucial for shakB(N)-specific rescue.

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