Fig. 5. Cenp-F localises to the nuclear periphery at the G2/M transition. Deconvolved images showing HeLa cells stained to detect Cenp-F (green) and DNA (blue). The cells were also stained to detect phospho-histone H3 (A,B) and Bub1 (C-E). Bars, 10 µm. (A) Interphase and prometaphase cells showing localisation of Cenp-F to the nucleus and kinetochores, respectively. (B) Two interphase cells; the one on the left is in late G2 and shows Cenp-F localising to the nucleus and the nuclear envelope. A small fraction is present in the cytoplasm. (C) Prometaphase cell showing colocalisation of Cenp-F and Bub1 at kinetochores. (D) Late prophase cell showing Cenp-F in the nucleus, cytoplasm and at the nuclear envelope. (E) A cell at the G2/M transition showing early signs of chromosome condensation (see inset), a few Bub1 foci and Cenp-F at the nuclear envelope.

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