Video sequence of CHOK1 cells co-transfected with GFP-vinculin and control vector. GFP-vinculin-containing focal adhesions are highly dynamic at the cell periphery.

Video sequence of CHOK1 cells co-transfected with GFP-vinculin and calpastatin. Inhibition of calpain, through the overexpression of calpastatin, decreases the disassembly of GFP-vinculin-containing focal complexes. GFP-vinculin-containing focal complexes are larger and more peripheral than focal complexes in control cells.

Video sequence of CHOK1 cells co-transfected with GFP-zyxin and calpastatin. Inhibition of calpain, through the overexpression of calpastatin, decreases the disassembly of GFP-zyxin-containing focal complex. GFP-zxyin-containing focal complexes are larger and more peripheral than focal complexes in control cells.

Video sequence of CHOK1 cells co-transfected with hrEGFP and RFP-zyxin. Similar to Movie 3, RFP-zyxin-containing focal adhesions are highly dynamic at the cell periphery.

Video sequence of CHOK1 cells co-transfected with hrEGFP-calpastatin and RFP-zyxin. Expression of GFP-calpastatin inhibits disassembly of RFP-zyxin-containing focal complexes. RFP-zyxin-containing focal complexes are larger and more peripheral than focal complexes in control cells.

Video sequence of CHOK1 cells co-transfected with GFP-a actinin and control vector. GFP-a-actinin is localized throughout the cell and is highly dynamic.

Video sequence of CHOK1 cells co-transfected with GFP-a-actinin and calpastatin. Inhibition of calpain, through the overexpression of calpastatin, disrupts the localization and the dynamics of GFP-a-actinin.

Video sequence of CHOK1 cells co-transfected with RFP-zyxin and GFP-a-actinin. Representative cells show the co-localization and dynamics of RFP-zyxin and GFP-a-actinin. Notably, RFP-zyxin and GFP-a-actinin are predominantly co-localized in the cells. Zyxin-containing focal complexes are highly dynamic and can be seen to disassemble or translocate to the cell center.

Video sequence of CHOK1 cells co-transfected with RFP-zyxin and GFP-a-actinin. Representative cells show the co-localization and dynamics of RFP-zyxin and GFP-a-actinin. Notably, RFP-zyxin and GFP-a-actinin are predominantly co-localized in the cells. Zyxin-containing focal complexes are highly dynamic and can be seen to disassemble or translocate to the cell center.

Video sequence of RFP-zyxin in CHOK1 cells co-transfected with full-length a-actinin. RFP-zyxin-containing focal complexes are highly dynamic in CHOK1 cells that express full-length a-actinin.

Video sequence of RFP-zyxin in CHOK1 cells co-transfected with the head domain of a-actinin. The head domain of a-actinin localizes primarily to the actin cytoskeleton. RFP-zyxin-containing focal complexes are dynamic in CHOK1 cells that express the head domain of a-actinin.

Video sequence of RFP-zyxin in CHOK1 cells co-transfected with the rod domain of a-actinin. The rod domain of a-actinin localizes primarily to focal complexes. RFP-zyxin-containing focal complexes are less dynamic and do not translocate to the cell center, which is similar to the effects observed after calpain inhibition.