Fig. 3. Effects of calpain inhibition on focal adhesion disassociation during nocodozole recovery in cells plated on fibronectin. (A) CAR fibroblasts were transfected with EGFP-zyxin. Cells were pretreated with nocodozole (a) or nocodozole and ALLN (b) and plated in the presence of the same agents on 50 µg/ml fibronectin for 2 hours. Nocodozole was then washed out. Cells were allowed to recover in the presence (b) or absence (a) of ALLN. In control cells contacts were disassembled as cells spread. In cells treated with ALLN, turnover of adhesive contact sites was inhibited. Bars, 10 µm. (B) Quantification of contact site disassembly or formation in control and ALLN-treated cells from four separate experiments.

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