Fig. 5. Effects of calpain inhibition on -actinin localization and dynamics in CHOK1 cells plated on fibronectin. (A) Representative images of fixed cells treated with (b) and without (a) ALLN, and fixed cells that have been transfected with hrEGFP (c) or hrEGFP-calpastatin (d). Arrows and inserts show GFP-positive cells. (B) Live imaging of cells co-transfected with GFP—-actinin and control vector (a), and cells co-transfected with GFP—-actinin and calpastatin (b). GFP—-actinin was less dynamic and did not localize to focal complexes in cells that expressed calpastatin in comparison to control vector (Movies 7, 8). Bars, 20 µm.

Return to article