Fig. 9. Effects of disrupting -actinin localization on focal adhesion dynamics. (A) Still images from video sequences of CHOK1 cells co-transfected with RFP-zyxin and full-length GFP—-actinin (a), -actinin-head—GFP (b) or -actinin-rod—GFP (c) (Movies 12, 13 and 14, respectively) RFP-zyxin-containing focal complexes are more peripheral and less dynamic when -actinin localization to the focal complex is disrupted by -actinin-rod—GFP, similar to calpain inhibition. In contrast, RFP-zyxin in -actinin-head—GFP co-transfected cells exhibits a focal adhesion distribution and dynamics similar to full-length GFP—-actinin (Movies 11-13). Bar, 20 µm. (B) Quantification of focal adhesion dynamics in cells co-transfected with full-length -actinin, -actinin-head—GFP, or -actinin-rod—GFP and RFP-zyxin. Transfection of -actinin-rod—GFP inhibits focal adhesion disassembly when compared with full-length -actinin (P<0.002) and with -actinin-head—GFP (P<0.003). Quantification of focal adhesion dynamics was performed on five cells for each condition.

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