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Fig. 3. Effect of the calcium ionophore A23187 on the sulfation of ricin sulf-2.
MDCK II cells were incubated with radioactive sulfate for 3 hours at 37°C,
and 10 µM A23187 was added for the last 30 minutes. Then ricin sulf-2
(
200 ng/ml) was added to the medium, and the incubation was continued for
2 hours. The cells were subsequently washed with 0.1 M lactose, lysed and
immunoprecipitated with rabbit anti-ricin antibodies. The adsorbed material
was analyzed by SDS-PAGE (12%) before autoradiography.
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