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Fig. 5. Effect of thapsigargin on the localization of ricin B-chain. MDCK II cells
grown on coverslips were incubated for 30 minutes at 37°C in the absence
(A-C) or presence of 1 µg/ml thapsigargin (D-F). Then CY3-labeled ricin
B-chain (1 µg/ml) (red) was added, and the incubation continued for 2
hours. The Golgi apparatus was labeled with sheep anti-human TGN46 antibodies
followed by donkey anti-sheep/goat IgG FITC (green). C and F represent the
merged images.
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