Fig. 8. Cell surface expression of myc-TRPC4 is altered by co-expression of the YFP-ERM mutant. Association of myc-TRPC4 with the plasma membrane in HEK293 cells expressing YFP-EBP50 or YFP-ERM was analyzed by cell surface biotinylation, as described in the legend to Fig. 5. (A) Representative western blots of total (left panel) and biotinylated proteins (right panel) extracted from HEK293 cells expressing myc-TRPC4 together with YFP-EBP50 or YFP-ERM. S_EBP50 corresponds to the slope of the linear fit obtained from the YFP-EBP50-expressing cells (filled circles, solid line) and S_ERM represents the slope of the linear fit derived from the YFP-ERM-expressing cells (open circles, dotted line). (B) Relative total (R_T) and surface (R_S) contents of myc-TRPC4, in YFP-EBP50- and YFP-ERM-expressing cells were calculated as described in the legend to Fig. 5. R_S/R_T was deduced from three blot pairs similar to those presented in A and is shown as mean±s.e.m.

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