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Fig. 2. Effects of L63-RhoA and N19-RhoA on stress-fibre formation. (A) Cells were
transfected with EGFP-L63-RhoA and then fixed, permeablised, stained for
F-actin with Alexa 546 phalloidin and examined by confocal microscopy. (B)
Cells were transfected with EGFP-N19-RhoA in the absence or presence of 40 nM
LTD4 for 5 minutes and then processed as in A. Merged images are
shown on the right. The results illustrated in this figure are representative
of six separate experiments.