Fig. 3. GFP-tagged MyBPs localize to the A-band of differentiating myotubes. Myoblasts from 11-day-old chick embryos were isolated, cultured and transfected the following day using lipofectamine with GFP/C, GFP/C7-10 or GFP/H. Five days after transfection, live cells expressing GFP-tagged MyBPs appeared striated and contractile when viewed with fluorescence optics. Cells were fixed and probed with F59, a mAb against sarcomeric myosin. GFP/C (B), GFP/C7-10 (E) and GFP/H (F) all incorporate into the A-bands of cultured embryonic muscle cells. F59 immunostaining revealed a similar myosin distribution to that seen with GFP/C (C). The overlap of GFP/C and myosin is shown in the D.

Return to article