Fig. 7. Interaction of MyBP-H truncation mutants with MyHC when co-expressed in COS cells. Expression of MyBP-H constructs without MyHC (A-D) or with MyHC (E-P). Cells were probed with biotinylated 9E10 and F59 to reveal myc-tagged MyBP-H recombinant proteins (A-H) and MyHC (I-L), respectively. The overlap of MyBP-H and MyHC is shown in M-P. As described in Fig. 1, the following MyBP-H constructs were used: full-length MyBP-H (A,E,I,M), HU (B,F,J,N), HU1 (C,G,K,O) and H4 (D,H,L,P). Each of the MyBP-H fragments co-distributed with MyHC in the cell (M-P). H4, which lacks the major myosin rod-binding domain, did not induce MyHC to form cables.

Return to article