Fig. 5. MT treadmilling and GFP-CLIP-170. MT dynamics and GFP-CLIP-170 movement were analysed in cytoplasts lacking the centrosome. (A) MTs and CLIP-170 images (two left pictures) and time lapse sequence of merged images with GFP-CLIP-170 set to the red channel and MTs to the green channel, respectively. Plus and minus ends of treadmilling MTs are indicated by arrowheads. CLIP-170 labels the plus ends of treadmilling MTs while minus ends are always CLIP-170-negative. Numbers in the top-left corner indicate time in seconds; bar, 5 µm. (B) Example histories of CLIP-170 tracks in cytoplasts lacking a centrosome. Individual plots are arbitrarily staggered along the time axis for clarity. Scale of axes is indicated in the top-right corner of the graph. (C) Life history plot of a treadmilling MT (plus and minus ends are green) and CLIP-170 (red). Time shift between plus end history and CLIP-170 history is 2.5 seconds. CLIP-170 persists at the growing plus end of the treadmilling MT.

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