Fig. 7. Other CAR components show a similar delay in recruitment to the medial cortex in TBZ. (A) Temperature-arrested myo2-gc cdc25-22 cells were released into either DMSO (left panels) or 100 µg/ml TBZ (right panels) and stained for actin. Whereas control cells at the 40 minute time point formed rings containing both actin (I,ii) and Myo2 (iii), TBZ-treated cells showed actin remaining at the cell poles and no Myo2 rings. Panel Aiv shows the merged images. (B) mid1-gfp cdc25-22 cells synchronised by transient temperature shift and released in the presence (open symbols) or absence (filled symbols) of 100 µg/ml TBZ. Whereas control cells formed normal Mid1-GFP rings (filled pink circles) followed by septa (filled black squares), both were substantially abolished in TBZ. (C) Control cells from the 40 minute time point in (B) showing Mid1 rings. (D) An equivalent sample from the TBZ-treated culture. In about 20% of TBZ-treated cells, Mid1-GFP localised to the medial cortex but failed to form a ring. Left panels in C and D: Mid1-GFP; right hand panels: DAPI-phase. Bar, 10 µm.

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