Fig. 9. Cdc7 localisation to the SPBs is co-commitant with Myo2 ring recruitment. myo2-gc cdc7-gfp cdc25-22 cells were synchronised by transient temperature shift and released in the absence (A, filled symbols) or presence (B, open symbols) of 100 µg/ml TBZ. In (A), Cdc7 fluorescence at the SPBs (red squares) is coincident with the appearance of Myo2 rings (green circles). Both precede the appearance of binucleate cells (blue diamonds). Both Cdc7 at the SPBs and Myo2 ring formation are delayed in the presence of TBZ. (C,D) Cells from the 40 minute time point in A and the 100 minute time point in B, respectively (left hand panels: Cdc7 and Myo2-GFP; right hand panels: DNA). Bar, 10 µm.

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