|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 6. MEK2-dependent JNK activation. (A) Immunoblots of RD cells transfected with
the constitutively active form of HA-tagged MEK2 (CA-MEK2) or with the empty
vector (CMV) using antibodies that recognize phospho-active ERKs and
hemagglutinin (HA). (B) A luciferase assay for detection of activated JNKs
(see Materials and Methods); luciferase activity (units/plates) was assayed in
total lysates from RD cells co-transfected with a constitutively active form
of MEK2 (CA-MEK2) or with empty vector (CMV) and both with activator plasmid
GAL4-Jun and reporter plasmid GAL4-luc. The data shown are representative of
two independent experiments.
|
