Fig. 7. Effects of anisomycin on JNK and p38 phosphorylations. (A) Immunoblots of total lysates of control (C) and TPA-treated cells both in the absence (TPA) and in the presence of 10 ng/ml anisomycin (AN, AN + TPA) for 30 minutes and 5 days using antibodies specific for phospho-active JNKs and p38. For normalization, filters were re-probed with antibodies that recognize total protein. (B) Immunoblots, using anti-sarcomeric MHC antibody, of total cells lysates from RD cells untreated (C) or treated with different doses of anisomycin (5, 10, 50 ng/ml, AN) for 3 days and chased for a further 3 days in the presence and in the absence of TPA. The data shown are representative of three different experiments.

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