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Fig. 4. Vinculin-positive adhesion plaques and hyaluronan at the cell-substratum
interface. (A) Double immunofluorescence for hyaluronan (green) and vinculin
(red) in confocal optical sections constituting the undersurface of the
keratinocytes. The image of vinculin is processed as for the quantification of
adhesion plaques, see Materials and Methods. (B) Quantification of
vinculin-positive areas by image analysis in the transfected cell lines. Means
and ranges of two separate experiments for each cell line are shown. One-way
analysis of variance and Fisher tests were used to indicate the cell lines
that were significantly different *(P<0.05) from mock
controls.
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