Fig. 7. Immunogold localization of Asy1 antibody to spread PMCs of B. oleracea. (A) Meiotic interphase showing localization of 5 nm gold particles to chromatin in the absence of axial elements. Linear arrays of gold particles are occasionally visible. Preparation stained with uranyl acetate. (B) Leptotene showing localization of 5 nm gold particles to chromatin associated with unsynapsed axial elements. The preparation was stained with osmium tetroxide. (C) Low power survey of pachytene chromosomes for orientation purposes; the hatched box indicates the region shown in (D). (D) Pachytene showing localization of 5 nm gold particles to chromatin associated with synaptonemal complex. Chromosome axes are indicated with arrows; the boxed area (line) shows an example of gold particles on chromatin (grey area) associated with the axes; the hatched box shows absence of gold particles on regions free of chromatin (white) Preparation stained with PTA. Bar, 200 nm.

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