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Fig. 2. Neuroepithelial cells and residual ES cells in non-selected ES-cell-derived
cultures. ES cells were cultured as shown in
Fig. 1 but without selection.
At various times, the cells were dissociated, allowed to settle on a coverslip
for 6 hours and stained for SSEA-1 or nestin by immunohistochemistry. The
proportion of stained cells was determined, and the results are shown as
mean±s.d. of three independent experiments.
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