Fig. 6. Timing of neural cell development and marker expression in ES-cell-derived cultures. Engineered ES cells were treated and selected as described in Fig. 1. At various times after the end of the selection (day 10), Olig2 and PDGFR expression were analysed by in situ hybridization; neurofilaments, NG2, O4 and GC were analysed by immunohistochemistry and MBP expression was assessed by RT-PCR. (A) Expression of neuronal and OPC markers. (B) Expression of neuronal and oligodendrocyte markers. (C) Expression of MBP mRNA. Results of typical experiments are shown, but the experiments were repeated three times with similar results.

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