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Fig. 2. Rap1 levels during growth of the pVEII-AS5'-1 transformant
in the absence of folate. Cells were harvested at the day indicated following
the removal of folate, and the total cellular protein was fractionated by
SDS-PAGE. Rap1 levels were determined by western blot analysis using a
Dictyostelium Rap1-specific antibody. The RasG levels were determined
with a RasG-specific antibody.
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