Fig. 3. Response to osmotic shock. (A) Ax-2 ([UNK],) and pVEII-AS5'-1 (,) cells were shaken in KK₂ buffer containing 0.4 M sorbitol ([UNK],) or 0.4 M sorbitol and 100 µM 8Br-cAMP (,) for the indicated time periods and then diluted and plated in association with K.oxytoca to determine cell viability. The number of plaques that were formed on the bacterial lawns were counted are expressed as a percentage of the zero time value and are the means±s.e. for two experiments. (B) Ax-2 ([UNK]), pVEII-AS5'-1 transformant () and pVEII-Rap1 (G12V) transformant () cells were shaken in KK₂ in the presence of 0.4 M sorbitol for the indicated times. Aliquots were then treated with 3.5% perchloric acid and the amount of cGMP determined. The results are the mean±s.d. of five experiments for Ax-2, three experiments for pVEII-AS5' and the mean±s.e. for two experiments for pVEII-Rap1(G12V).

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