Fig. 5. MBSA-MDP induced the transport of Salmonella to the lysosomes. (A) To determine the nature of mature LSP, LSP were purified from untreated cells as well as from MBSA-MDP-treated cells as described in the Materials and Methods after 90 minutes of internalization in J774E cells. LSP were washed and the content of actin, cathepsin D and V-ATPase on respective LSP (40 µg each) was determined by western blot analysis using specific antibodies. Results from western blots are representative of three independent preparations. (B) Confocal images showing the GFP-Salmonella colocalization with Lysotracker-Red-labelled lysosomes in untreated (a-c) and MBSA-MDP-treated J774E cells (d-f). Untreated or MBSA-MDP-treated J774E cells were infected with GFP-Salmonella typhimurium and chased for 90 minutes to determine their colocalization with Lysotracker-Red-labelled lysosomes as described in the Materials and Methods. Lysotracker-Red-labelled lysosomes appear in the red channel (a,d); green channel shows the GFP-Salmonella (b,e); and yellow indicates the colocalization of GFP-Salmonella with Lysotracker-labelled lysosomes in merged images (c,f).

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